Trypsin is a kind of digestive enzyme found in pancreas and used for antiflatulents and amino acid sequence analysis.
The enzyme activity is determined based on the change in the absorbance per time unit when an enzyme decomposes a substrate. The larger the change in the absorbance (slope), the higher the enzyme activity. When the activity of a commercially available trypsin was calculated, it was found to be 3700 trypsin unit/mg indicating that the value claimed by the manufacturer (3500 trypsin unit/mg or higher) was satisfied. The activity level can be easily calculated by using the rate calculation function.
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